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Anti-Rabies virus/RABV G/Glycoprotein Polyclonal Antibody, Biotin (VK632324)

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Overview
Catalog No.VK632324
Description
Anti-Rabies virus/RABV G/Glycoprotein Polyclonal Antibody, Biotin (VK632324) is a rabbit polyclonal antibody detecting Glycoprotein in WB, IHC, ELISA.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Biotin Conjugated — Signal amplification with streptavidin systems.
Species reactivityRabies virus (RABV)
ApplicationsWB, IHC, ELISA
Host speciesRabbit
IsotypeIgG
ConjugationBiotin
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rabies virus/RABV G/Glycoprotein (Leu30-Tyr459).
Target G, Glycoprotein
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession O92284
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycoprotein is a ~58 kDa protein. Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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