Anti-MPK6 Polyclonal Antibody (PR006014)

Name: Anti-MPK6 Polyclonal Antibody
Brand: abinScience
SKU: PR006014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

PR006014

Description

Anti-MPK6 Polyclonal Antibody (PR006014) is a rabbit polyclonal antibody detecting MPK6 in ELISA, IHC, WB. Suitable for Nicotiana tabacum, Medicago sativa, Pisum sativum, and Capsicum annuum.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Arabidopsis thaliana (Mouse-ear cress), Nicotiana tabacum (Common tobacco), Oryza sativa (Rice)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant MPK6 (Asn35-Gln395).

Target

Mitogen-activated protein kinase 6, AtMPK6, MAP kinase 6, EC:2.7.11.24, MPK6

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q39026, Q40532, Q07176, A0A1S3Z5Y0, Q06060, Q9LKZ1, Q84UI5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
WB	1:500-1:2000
IHC	1:50-1:500

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase 6 (MPK6) is a ~45 kDa protein. Mitogen-activated protein kinase (MAPK) which regulates abscisic acid (ABA) responses in a MAPKKK20-MKK5-MPK6 cascade involved in root growth (e.g. root cell division and elongation) and stomatal response. Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking.

1. Li, K. et al. (2017) Plant physiology 173, 1391-1408. PMID: 27913741

2. Zhang, Y. et al. (2015) Developmental cell 33, 136-49. PMID: 25843888

3. Forzani, C. et al. (2011) The FEBS journal 278, 1126-36. PMID: 21276203

Note

For research use only

Images

References

Datasheet

Anti-MPK6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MPK6 Polyclonal Antibody (PR006014)

Overview

Images

References

Datasheet

SDS

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