Anti-Human CD86/B7-2 Recombinant Antibody (3D1) (HW776023)

Name: Anti-Human CD86/B7-2 Recombinant Antibody (3D1)
Brand: abinScience
SKU: HW776023
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW776023

Species reactivity

Porcine

Applications

ELISA, FCM, IP

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

3D1

Clonality

Monoclonal

Target

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2, B7-2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P42081, K7GMU1

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-lymphocyte activation antigen CD86 (B7-2) is a ~37 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.

1. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

2. Freeman, GJ. et al. (1993) Science (New York, N.Y.) 262, 909-11. PMID: 7694363

3. Lanier, LL. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 97-105. PMID: 7527824

4. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD86/B7-2 (Catalog No: HW776011)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 monoclonal antibody (Catalog HW776023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD86/B7-2 Recombinant Antibody (3D1).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CD86/B7-2 Recombinant Antibody (3D1) (HW776023)

Overview

Images

References

Datasheet

SDS

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