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Anti-Mouse CHIL3 Polyclonal Antibody (MA579014)

Anti-Mouse CHIL3 Polyclonal Antibody
Anti-Mouse CHIL3 Polyclonal Antibody
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Overview
Catalog No.MA579014
Description
Anti-Mouse CHIL3 Polyclonal Antibody (MA579014) is a rabbit polyclonal antibody detecting Chil3 in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse CHIL3 (Tyr22-Tyr398).
Target Chitinase-like protein 3, EC:3.2.1.52, Beta-N-acetylhexosaminidase Ym1, Chitinase-3-like protein 3, ECF-L, Eosinophil chemotactic cytokine, Secreted protein Ym1, Chil3, Chi3l3, Ym1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O35744
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Chitinase-like protein 3 (CHIL3) is a ~44 kDa protein. Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils.

Note For research use only
Images
  • Anti-Mouse CHIL3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CHIL3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse spleen lysate
    Lane 2: Mouse lung lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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