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Anti-SURF4 Polyclonal Antibody (HA590014)

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Overview
Catalog No.HA590014
Description
Anti-SURF4 Polyclonal Antibody (HA590014) is a rabbit polyclonal antibody detecting SURF4 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Zebrafish.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human SURF4 (Gly2-Tyr63&Asp113-Ser178).
Target Surfeit locus protein 4, SURF4, SURF-4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O15260, Q64310, A7YY49, Q800K9, Q7SZQ7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Surfeit locus protein 4 (SURF4) is a ~30 kDa protein. Endoplasmic reticulum cargo receptor that mediates the export of lipoproteins by recruiting cargos into COPII vesicles to facilitate their secretion. Acts as a cargo receptor for lipoproteins bearing both APOB and APOA1, thereby regulating lipoprotein delivery and the maintenance of lipid homeostasis. Synergizes with the GTPase SAR1B to mediate transport of circulating lipoproteins. Promotes the secretion of PCSK9. Also mediates the efficient secretion of erythropoietin (EPO).

1. Saegusa, K. et al. (2018) The Journal of cell biology 217, 2073-2085. PMID: 29643117
2. Emmer, BT. et al. (2018) eLife 7. PMID: 30251625
3. Wang, X. et al. (2021) Cell metabolism 33, 350-366.e7. PMID: 33186557
4. Lin, Z. et al. (2020) Molecular and cellular biology 40. PMID: 32989016
5. Mitrovic, S. et al. (2008) Molecular biology of the cell 19, 1976-90. PMID: 18287528
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SURF4 Polyclonal Antibody

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