Anti-Human CD371/CLEC12A Polyclonal Antibody (HV493014)

Name: Anti-Human CD371/CLEC12A Polyclonal Antibody
Brand: abinScience
SKU: HV493014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV493014

Description

Anti-Human CD371/CLEC12A Polyclonal Antibody (HV493014) is a rabbit polyclonal antibody detecting CLEC12A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD371/CLEC12A (Arg136-Ala265).

Target

C-type lectin domain family 12 member A, C-type lectin-like molecule 1, CLL-1, Dendritic cell-associated lectin 2, DCAL-2, Killer cell C-type lectin-like receptor L1, hKLRL1, Myeloid inhibitory C-type lectin-like receptor, MICL, CD371, CLEC12A, CLL1, DCAL2, KLRL1, MICL

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q5QGZ9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

C-type lectin domain family 12 member A (CD371/CLEC12A) is a ~30 kDa protein. Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation. Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation. Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals. Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid. Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation.

1. Marshall, AS. et al. (2004) The Journal of biological chemistry 279, 14792-802. PMID: 14739280

2. Han, Y. et al. (2004) Blood 104, 2858-66. PMID: 15238421

3. Chen, CH. et al. (2006) Blood 107, 1459-67. PMID: 16239426

4. Paré, G. et al. (2021) Frontiers in immunology 12, 650808. PMID: 34234773

5. Tang, H. et al. (2024) The Journal of biological chemistry 300, 105765. PMID: 38367667

6. Mori, S. et al. (2024) International immunology 36, 279-290. PMID: 38386511

7. Malamud, M. et al. (2024) Nature 633, 442-450. PMID: 39143217

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-Human CD371/CLEC12A polyclonal antibody at 1ug/mL on recombinant Human CD371/CLEC12A (Catalog No. HV493012).

References

Datasheet