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Anti-CHIKV Capsid Protein Polyclonal Antibody (VK572024)

Anti-CHIKV Capsid Protein Polyclonal Antibody
Anti-CHIKV Capsid Protein Polyclonal Antibody
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Overview
Catalog No.VK572024
Description
Anti-CHIKV Capsid Protein Polyclonal Antibody (VK572024) is a rabbit polyclonal antibody detecting Capsid protein in ELISA, IHC, WB. Suitable for Chikungunya virus, O'nyong-nyong virus, Middelburg virus, Ross river virus, and Sagiyama virus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityChikungunya virus (CHIKV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant CHIKV Capsid Protein (Arg104-Trp261).
Target C, Capsid protein, Coat protein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P0DOK1, Q8JUX5, Q5WQY5, Q5XXP3, O90371, P22056, O90369, P03315, P0DJZ6, Q80S27, P08491, Q9JGK8, Q5Y388, P13890, Q8QZ72
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Frameshifted structural polyprotein is a ~92 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

Note For research use only
Images
  • Anti-CHIKV Capsid Protein Polyclonal Antibody

    Western blot

    Lane 1: recombinant CHIKV Capsid (Catalog No: VK572022)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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