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Anti-CHIKV NSP1 Polyclonal Antibody (VK640024)

Anti-CHIKV NSP1 Polyclonal Antibody
Anti-CHIKV NSP1 Polyclonal Antibody
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  • 100ug
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Overview
Catalog No.VK640024
Description
Anti-CHIKV NSP1 Polyclonal Antibody (VK640024) is a rabbit polyclonal antibody detecting mRNA-capping enzyme nsP1 in ELISA, IHC, WB. Suitable for Chikungunya virus and O'nyong-nyong virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityChikungunya virus (CHIKV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant CHIKV Polyprotein P1234 (Met1-Gln364).
Target Polyprotein P1234, P1234, Non-structural polyprotein, Polyprotein P123, P123, mRNA-capping enzyme nsP1, EC:2.1.1.-, EC:2.7.7.-, Non-structural protein 1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession Q8JUX6, Q5XXP4, O90368, P13886, O90370
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Polyprotein P1234 (nsp1) is a ~275 kDa protein. Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Note For research use only
Images
  • Anti-CHIKV NSP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NSP1-CHPV polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant CHIKV NSP1 (Catalog No: VK640022)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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