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Anti-TCF7 Polyclonal Antibody (HW608024)

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Overview
Catalog No.HW608024
Description
Anti-TCF7 Polyclonal Antibody (HW608024) is a rabbit polyclonal antibody detecting Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1; TCF7; TCF1 in ELISA, IHC, WB. Suitable for Mouse, Human, Zebrafish, Frog, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human TCF7 (Lys270-Glu355).
Target Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1; TCF7; TCF1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q00417, P36402, Q9NQB0, Q924A0, Q9YHE8, P70063, P70062, Q90ZB6, Q800Q5, P70064, P27782, Q9QXN1, Q9UJU2, Q9HCS4, Q9Z1J1, Q8IMA8, P91943
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Transcription factor 7 (TCF7) is a ~45 kDa protein. Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages.

1. Melichar, HJ. et al. (2007) Science (New York, N.Y.) 315, 230-3. PMID: 17218525
2. Malhotra, N. et al. (2013) Immunity 38, 681-93. PMID: 23562159
3. Spidale, NA. et al. (2018) Immunity 49, 857-872.e5. PMID: 30413363
Note For research use only
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Datasheet
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Formula
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Anti-TCF7 Polyclonal Antibody

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