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Anti-ID4 Polyclonal Antibody (HA666014)

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Overview
Catalog No.HA666014
Description
Anti-ID4 Polyclonal Antibody (HA666014) is a rabbit polyclonal antibody detecting DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; bHLHb27; Inhibitor of DNA binding 4; Inhibitor of differentiation 4; ID4; BHLHB27 in ELISA, IHC, WB. Suitable for Human, Pig, and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human ID4 (Lys2-Arg161).
Target DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; bHLHb27; Inhibitor of DNA binding 4; Inhibitor of differentiation 4; ID4; BHLHB27
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P47928, Q06AV5, P41139
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA-binding protein inhibitor ID-4 (ID4) is a ~16 kDa protein. Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.

Note For research use only
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Formula
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Formula
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Working Solution
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