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Anti-NUP214 Polyclonal Antibody (HA667014)

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Overview
Catalog No.HA667014
Description
Anti-NUP214 Polyclonal Antibody (HA667014) is a rabbit polyclonal antibody detecting Nuclear pore complex protein Nup214; 214 kDa nucleoporin; Nucleoporin Nup214; Protein CAN; NUP214; CAIN; CAN; KIAA0023 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human NUP214 (Met1-Asn407).
Target Nuclear pore complex protein Nup214; 214 kDa nucleoporin; Nucleoporin Nup214; Protein CAN; NUP214; CAIN; CAN; KIAA0023
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P35658, Q80U93
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nuclear pore complex protein Nup214 is a ~213 kDa protein. Part of the nuclear pore complex. Has a critical role in nucleocytoplasmic transport. May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.

1. Fornerod, M. et al. (1997) The EMBO journal 16, 807-16. PMID: 9049309
2. Fichtman, B. et al. (2019) American journal of human genetics 105, 48-64. PMID: 31178128
3. Kraemer, D. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 1519-23. PMID: 8108440
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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