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Anti-POLR2L Polyclonal Antibody (HA733014)

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Overview
Catalog No.HA733014
Description
Anti-POLR2L Polyclonal Antibody (HA733014) is a rabbit polyclonal antibody detecting and III subunit ABC5; DNA-directed RNA polymerase III subunit L; RNA polymerase II 7.6 kDa subunit; RPB7.6; RPB10 homolog; POLR2L in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Fruit fly.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human POLR2L (Ile2-Lys67).
Target DNA-directed RNA polymerases I, II, and III subunit RPABC5; RNA polymerases I, II, and III subunit ABC5; DNA-directed RNA polymerase III subunit L; RNA polymerase II 7.6 kDa subunit; RPB7.6; RPB10 homolog; POLR2L
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P62876, Q32P78, P62875, Q9VC49
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA-directed RNA polymerases I, II, and III subunit RPABC5 (POLR2L) is a ~7 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-POLR2L Polyclonal Antibody

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