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Anti-TLE5 Polyclonal Antibody (HA755014)

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Overview
Catalog No.HA755014
Description
Anti-TLE5 Polyclonal Antibody (HA755014) is a rabbit polyclonal antibody detecting transcriptional modulator; TLE5; AES; GRG; GRG5 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Frog.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human TLE5 (Met2-Asp197).
Target TLE family member 5; Amino-terminal enhancer of split; Amino enhancer of split; Gp130-associated protein GAM; Grg-5; Groucho-related protein 5; Protein ESP1; Protein GRG; TLE family member 5, transcriptional modulator; TLE5; AES; GRG; GRG5
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q08117, P63003, P63002, O42470
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

TLE family member 5 (TLE5) is a ~21 kDa protein. Transcriptional corepressor. Acts as a dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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