Anti-ERCC8 Polyclonal Antibody (HA782014)

Name: Anti-ERCC8 Polyclonal Antibody
Brand: abinScience
SKU: HA782014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HA782014

Description

Anti-ERCC8 Polyclonal Antibody (HA782014) is a rabbit polyclonal antibody detecting DNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA; ERCC8; CKN1; CSA in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human ERCC8 (Met1-Pro370).

Target

DNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA; ERCC8; CKN1; CSA

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q13216, Q5BIM8, Q8CFD5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA excision repair protein ERCC-8 (ERCC8) is a ~44 kDa protein. Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment. The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ).

1. Groisman, R. et al. (2003) Cell 113, 357-67. PMID: 12732143

2. Groisman, R. et al. (2006) Genes & development 20, 1429-34. PMID: 16751180

3. Angers, S. et al. (2006) Nature 443, 590-3. PMID: 16964240

4. Nakazawa, Y. et al. (2020) Cell 180, 1228-1244.e24. PMID: 32142649

5. Kokic, G. et al. (2021) Nature 598, 368-372. PMID: 34526721

6. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879

7. Carnie, CJ. et al. (2024) Nature cell biology 26, 797-810. PMID: 38600235

8. van Sluis, M. et al. (2024) Nature cell biology 26, 770-783. PMID: 38600236

9. van der Weegen, Y. et al. (2020) Nature communications 11, 2104. PMID: 32355176

Note

For research use only

Images

References

Datasheet

Anti-ERCC8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-ERCC8 Polyclonal Antibody (HA782014)

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Datasheet

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