Anti-POLA2 Polyclonal Antibody (HA796014)

Name: Anti-POLA2 Polyclonal Antibody
Brand: abinScience
SKU: HA796014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HA796014

Description

Anti-POLA2 Polyclonal Antibody (HA796014) is a rabbit polyclonal antibody detecting DNA polymerase alpha subunit B; DNA polymerase alpha 70 kDa subunit; POLA2 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human POLA2 (Lys212-Ile598).

Target

DNA polymerase alpha subunit B; DNA polymerase alpha 70 kDa subunit; POLA2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q14181, Q58D13, O89043, P33611

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase alpha subunit B (POLA2) is a ~65 kDa protein. Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively.

1. Schneider, A. et al. (1998) The Journal of biological chemistry 273, 21608-15. PMID: 9705292

Note

For research use only

Images

References

Datasheet

Anti-POLA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-POLA2 Polyclonal Antibody (HA796014)

Overview

Images

References

Datasheet

SDS

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