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Anti-CDKAL1 Polyclonal Antibody (HB302014)

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Overview
Catalog No.HB302014
Description
Anti-CDKAL1 Polyclonal Antibody (HB302014) is a rabbit polyclonal antibody detecting Threonylcarbamoyladenosine tRNA methylthiotransferase; 2.8.4.5; CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase; CDKAL1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human CDKAL1 (Gln64-Pro500).
Target Threonylcarbamoyladenosine tRNA methylthiotransferase; 2.8.4.5; CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase; CDKAL1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5VV42, Q91WE6, Q6NS26
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Threonylcarbamoyladenosine tRNA methylthiotransferase (CDKAL1) is a ~65 kDa protein. Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-CDKAL1 Polyclonal Antibody

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