Anti-UFM1 Polyclonal Antibody (HB333014)

Name: Anti-UFM1 Polyclonal Antibody
Brand: abinScience
SKU: HB333014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB333014

Description

Anti-UFM1 Polyclonal Antibody (HB333014) is a rabbit polyclonal antibody detecting Ubiquitin-fold modifier 1; UFM1; C13orf20; BM-002 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Rat, and Zebrafish.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human UFM1 (Ser2-Gly83).

Target

Ubiquitin-fold modifier 1; UFM1; C13orf20; BM-002

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P61960, P61961, Q2KJG2, Q5BJP3, Q803Y4, Q5ZMK7, Q5RJW4, B5LVL2, P34661, A8DYH2, Q9CA23

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin-fold modifier 1 (UFM1) is a ~9 kDa protein. Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer. The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1. Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.

1. Komatsu, M. et al. (2004) The EMBO journal 23, 1977-86. PMID: 15071506

2. Tatsumi, K. et al. (2010) The Journal of biological chemistry 285, 5417-27. PMID: 20018847

3. Oweis, W. et al. (2016) Cell reports 16, 3113-3120. PMID: 27653677

4. Nahorski, MS. et al. (2018) Brain : a journal of neurology 141, 1934-1945. PMID: 29868776

5. Walczak, CP. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 1299-1308. PMID: 30626644

6. He, C. et al. (2024) Molecular cell 84, 1120-1138.e8. PMID: 38377992

7. DaRosa, PA. et al. (2024) Nature 627, 445-452. PMID: 38383785

8. Yoo, HM. et al. (2014) Molecular cell 56, 261-274. PMID: 25219498

9. Liang, JR. et al. (2020) Cell 180, 1160-1177.e20. PMID: 32160526

Note

For research use only

Images

References

Datasheet

Anti-UFM1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-UFM1 Polyclonal Antibody (HB333014)

Overview

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References

Datasheet

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