Anti-LCP2 Polyclonal Antibody (HB352014)

Name: Anti-LCP2 Polyclonal Antibody
Brand: abinScience
SKU: HB352014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HB352014

Description

Anti-LCP2 Polyclonal Antibody (HB352014) is a rabbit polyclonal antibody detecting Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76; LCP2 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human LCP2 (Pro408-Pro533).

Target

Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76; LCP2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q13094, Q60787

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lymphocyte cytosolic protein 2 (LCP2) is a ~60 kDa protein. Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells. Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK. In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation. Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses.

1. Herndon, TM. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5654-64. PMID: 11313406

2. Soini, L. et al. (2021) FEBS letters 595, 404-414. PMID: 33159816

3. Wu, J. et al. (1996) Immunity 4, 593-602. PMID: 8673706

4. Bubeck Wardenburg, J. et al. (1996) The Journal of biological chemistry 271, 19641-4. PMID: 8702662

5. Yan, Z. et al. (2021) Nature communications 12, 308. PMID: 33436632

Note

For research use only

Images

References

Datasheet

Anti-LCP2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-LCP2 Polyclonal Antibody (HB352014)

Overview

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References

Datasheet

SDS

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