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Anti-SF3A3 Polyclonal Antibody (HB387014)

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Overview
Catalog No.HB387014
Description
Anti-SF3A3 Polyclonal Antibody (HB387014) is a rabbit polyclonal antibody detecting Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human SF3A3 (Met1-Leu501).
Target Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q12874, Q9D554
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Splicing factor 3A subunit 3 (SF3A3) is a ~58 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.

1. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114
2. Nesic, D. et al. (2001) Molecular and cellular biology 21, 6406-17. PMID: 11533230
3. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006
4. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310
5. Chiara, MD. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 6403-7. PMID: 8022796
6. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106
7. Zhan, X. et al. (2018) Cell research 28, 1129-1140. PMID: 30315277
Note For research use only
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Formula
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Anti-SF3A3 Polyclonal Antibody

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