Anti-UBE2A Polyclonal Antibody (HB413014)

Name: Anti-UBE2A Polyclonal Antibody
Brand: abinScience
SKU: HB413014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB413014

Description

Anti-UBE2A Polyclonal Antibody (HB413014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A in ELISA, IHC, WB. Suitable for Human, Mouse, Rabbit, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human UBE2A (Met1-Cys152).

Target

Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P49459, Q9Z255, P63146, P63148, P63149, P63147, Q32P99, P25153, P52478

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin-conjugating enzyme E2 A (UBE2A) is a ~17 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN.

1. Kim, J. et al. (2005) Molecular cell 20, 759-70. PMID: 16337599

2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

3. Haddad, DM. et al. (2013) Molecular cell 50, 831-43. PMID: 23685073

4. Hong, JH. et al. (2015) Molecular & cellular proteomics : MCP 14, 674-85. PMID: 25582440

5. Haakonsen, DL. et al. (2024) Nature 626, 874-880. PMID: 38297121

6. Barnsby-Greer, L. et al. (2024) Nature structural & molecular biology 31, 351-363. PMID: 38182926

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-UBE2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: A375 cell lysate
Lane 5: SW620 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: N2A cell lysate
Lane 8: NIH3T3 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Rat brain lysate

References

Datasheet

Anti-UBE2A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-UBE2A Polyclonal Antibody (HB413014)

Overview

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References

Datasheet

SDS

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