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Anti-UBE2R2 Polyclonal Antibody (HB434014)

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Overview
Catalog No.HB434014
Description
Anti-UBE2R2 Polyclonal Antibody (HB434014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 R2; 2.3.2.23; E2 ubiquitin-conjugating enzyme R2; Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2; UBE2R2; CDC34B; UBC3B in ELISA, IHC, WB. Suitable for Mouse, Human, and Rabbit.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human UBE2R2 (Met1-Cys191).
Target Ubiquitin-conjugating enzyme E2 R2; 2.3.2.23; E2 ubiquitin-conjugating enzyme R2; Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2; UBE2R2; CDC34B; UBC3B
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6ZWZ2, Q712K3, Q29503, P49427, Q8CFI2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-conjugating enzyme E2 R2 (UBE2R2) is a ~27 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. Works in collaboration with various Cul1-RING and Cul2-RING E3 ligase complexes. May be involved in degradation of katenin.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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