Anti-SNRPD3 Polyclonal Antibody (HB460014)

Name: Anti-SNRPD3 Polyclonal Antibody
Brand: abinScience
SKU: HB460014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HB460014

Description

Anti-SNRPD3 Polyclonal Antibody (HB460014) is a rabbit polyclonal antibody detecting Small nuclear ribonucleoprotein Sm D3; Sm-D3; snRNP core protein D3; SNRPD3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Frog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human SNRPD3 (Met1-Arg126).

Target

Small nuclear ribonucleoprotein Sm D3; Sm-D3; snRNP core protein D3; SNRPD3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P62318, P62320, P62323

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Small nuclear ribonucleoprotein Sm D3 (SNRPD3) is a ~13 kDa protein. Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638

2. Chari, A. et al. (2008) Cell 135, 497-509. PMID: 18984161

3. Pomeranz Krummel, DA. et al. (2009) Nature 458, 475-80. PMID: 19325628

4. Kondo, Y. et al. (2015) eLife 4. PMID: 25555158

5. Agafonov, DE. et al. (2016) Science (New York, N.Y.) 351, 1416-20. PMID: 26912367

6. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346

7. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770

8. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

9. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-SNRPD3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Caco2 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Rat brain lysate
Lane 8: Rat liver lysate

References

Datasheet

Anti-SNRPD3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-SNRPD3 Polyclonal Antibody (HB460014)

Overview

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References

Datasheet

SDS

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