Anti-GPS2 Polyclonal Antibody (HB469014)

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Overview

Catalog No.

HB469014

Description

Anti-GPS2 Polyclonal Antibody (HB469014) is a rabbit polyclonal antibody detecting G protein pathway suppressor 2; GPS-2; GPS2 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human GPS2 (Pro2-Lys327).

Target

G protein pathway suppressor 2; GPS-2; GPS2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q13227, Q921N8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

G protein pathway suppressor 2 (GPS2) is a ~36 kDa protein. Key regulator of inflammation, lipid metabolism and mitochondrion homeostasis that acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, thereby inhibiting 'Lys-63'-linked ubiquitination. In the nucleus, can both acts as a corepressor and coactivator of transcription, depending on the context. Acts as a transcription coactivator in adipocytes by promoting the recruitment of PPARG to promoters: acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, leading to stabilization of KDM4A and subsequent histone H3 'Lys-9' (H3K9) demethylation. Promotes cholesterol efflux by acting as a transcription coactivator. Acts as a regulator of B-cell development by inhibiting UBE2N/Ubc13, thereby restricting the activation of Toll-like receptors (TLRs) and B-cell antigen receptors (BCRs) signaling pathways.

1. Bi, H. et al. (2014) Molecular biology of the cell 25, 2499-508. PMID: 24943844

2. Jakobsson, T. et al. (2009) Molecular cell 34, 510-8. PMID: 19481530

3. Cardamone, MD. et al. (2018) Molecular cell 69, 757-772.e7. PMID: 29499132

4. Cheng, X. et al. (2009) The Journal of biological chemistry 284, 36395-36404. PMID: 19858209

5. Venteclef, N. et al. (2010) Genes & development 24, 381-95. PMID: 20159957

6. Spain, BH. et al. (1996) Molecular and cellular biology 16, 6698-706. PMID: 8943324

7. Huang, XD. et al. (2016) Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 37, 13333-13343. PMID: 27460081

Note

For research use only

Images

References

Datasheet

Anti-GPS2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-GPS2 Polyclonal Antibody (HB469014)

Overview

Images

References

Datasheet

SDS

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