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Anti-RNF19B Polyclonal Antibody (HB475014)

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Overview
Catalog No.HB475014
Description
Anti-RNF19B Polyclonal Antibody (HB475014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase RNF19B; 2.3.2.31; IBR domain-containing protein 3; Natural killer lytic-associated molecule; RING finger protein 19B; RNF19B; IBRDC3; NKLAM in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human RNF19B (Ala114-Ser323).
Target E3 ubiquitin-protein ligase RNF19B; 2.3.2.31; IBR domain-containing protein 3; Natural killer lytic-associated molecule; RING finger protein 19B; RNF19B; IBRDC3; NKLAM
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6ZMZ0, A2A7Q9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

E3 ubiquitin-protein ligase RNF19B is a ~77 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Protects against staurosporin-induced cell death.

1. Fortier, JM. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 6454-63. PMID: 16709802
2. Kaneko, M. et al. (2016) Scientific reports 6, 30955. PMID: 27485036
3. Kozlowski, M. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1775-85. PMID: 10438909
Note For research use only
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Formula
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