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Anti-GHITM Polyclonal Antibody (HB496014)

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Overview
Catalog No.HB496014
Description
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human GHITM (Leu2-Arg82).
Target Growth hormone-inducible transmembrane protein; Dermal papilla-derived protein 2; Mitochondrial morphology and cristae structure 1; MICS1; Transmembrane BAX inhibitor motif-containing protein 5; GHITM; DERP2; MICS1; TMBIM5; My021; UNQ244/PRO281
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9H3K2, Q91VC9, Q5XIA8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Growth hormone-inducible transmembrane protein (GHITM) is a ~37 kDa protein. Plays an important role in maintenance of mitochondrial morphology and in mediating either calcium or potassium/proton antiport. Mediates proton-dependent calcium efflux from mitochondrion. Also functions as an electroneutral mitochondrial proton/potassium exchanger. Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c.

1. Oka, T. et al. (2008) Molecular biology of the cell 19, 2597-608. PMID: 18417609
2. Seitaj, B. et al. (2020) Cells 9. PMID: 32977469
3. Patron, M. et al. (2022) The EMBO journal 41, e110476. PMID: 35912435
4. Austin, S. et al. (2022) EMBO reports 23, e54978. PMID: 36321428
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-GHITM Polyclonal Antibody

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