Anti-SF3B4 Polyclonal Antibody (HB498014)

Name: Anti-SF3B4 Polyclonal Antibody
Brand: abinScience
SKU: HB498014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB498014

Description

Anti-SF3B4 Polyclonal Antibody (HB498014) is a rabbit polyclonal antibody detecting Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49; SAP 49; SF3B4; SAP49 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human SF3B4 (Met1-Arg206).

Target

Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49; SAP 49; SF3B4; SAP49

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q15427, Q8QZY9, Q6AYL5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Splicing factor 3B subunit 4 (SF3B4) is a ~44 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

1. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

2. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

3. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

4. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

Note

For research use only

Images

References

Datasheet

Anti-SF3B4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Molecular Weight *

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-SF3B4 Polyclonal Antibody (HB498014)

Overview

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References

Datasheet

SDS

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