Anti-DNASE1L3 Polyclonal Antibody (HB541014)

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Datasheet
SDS

Overview

Catalog No.

HB541014

Description

Anti-DNASE1L3 Polyclonal Antibody (HB541014) is a rabbit polyclonal antibody detecting Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3 in ELISA, IHC, WB. Suitable for Human and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human DNASE1L3 (Met21-Ser305).

Target

Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q13609, O89107

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Deoxyribonuclease gamma (DNASE1L3) is a ~35 kDa protein. Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells.

1. Yakovlev, AG. et al. (2000) The Journal of biological chemistry 275, 21302-8. PMID: 10807908

2. Shiokawa, D. et al. (1998) Apoptosis : an international journal on programmed cell death 3, 89-95. PMID: 14646506

3. Sisirak, V. et al. (2016) Cell 166, 88-101. PMID: 27293190

4. Zeng, Z. et al. (1997) Biochemical and biophysical research communications 231, 499-504. PMID: 9070308

5. Baron, WF. et al. (1998) Gene 215, 291-301. PMID: 9714828

6. Errami, Y. et al. (2013) The Journal of biological chemistry 288, 3460-8. PMID: 23229555

7. Mizuta, R. et al. (2013) PloS one 8, e80223. PMID: 24312463

Note

For research use only

Images

References

Datasheet

Anti-DNASE1L3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-DNASE1L3 Polyclonal Antibody (HB541014)

Overview

Images

References

Datasheet

SDS

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