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Anti-SLC27A4 Polyclonal Antibody (HV816014)

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Overview
Catalog No.HV816014
Description
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human SLC27A4 (Thr75-Leu643).
Target Long-chain fatty acid transport protein 4; FATP-4; Fatty acid transport protein 4; Arachidonate--CoA ligase; 6.2.1.15; Long-chain-fatty-acid--CoA ligase; 6.2.1.3; Solute carrier family 27 member 4; Very long-chain acyl-CoA synthetase 4; ACSVL4; 6.2.1.-; SLC27A4; ACSVL4; FATP4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6P1M0, Q91VE0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Long-chain fatty acid transport protein 4 (SLC27A4) is a ~72 kDa protein. Mediates the levels of long-chain fatty acids (LCFA) in the cell by facilitating their transport across cell membranes. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis.

1. Stahl, A. et al. (1999) Molecular cell 4, 299-308. PMID: 10518211
2. Gimeno, RE. et al. (2003) The Journal of biological chemistry 278, 16039-44. PMID: 12556534
3. Zhou, W. et al. (2010) Journal of biomolecular screening 15, 488-97. PMID: 20448275
4. Mitchell, RW. et al. (2011) Journal of neurochemistry 117, 735-46. PMID: 21395585
5. Krammer, J. et al. (2011) International journal of medical sciences 8, 599-614. PMID: 22022213
6. Ohkuni, A. et al. (2013) Biochemical and biophysical research communications 442, 195-201. PMID: 24269233
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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