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Anti-SEC16A Polyclonal Antibody (HB575014)

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Overview
Catalog No.HB575014
Description
Anti-SEC16A Polyclonal Antibody (HB575014) is a rabbit polyclonal antibody detecting Protein transport protein Sec16A; SEC16 homolog A; p250; SEC16A; KIAA0310; SEC16; SEC16L in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human SEC16A (Ser1612-Gly1890).
Target Protein transport protein Sec16A; SEC16 homolog A; p250; SEC16A; KIAA0310; SEC16; SEC16L
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O15027, E9QAT4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein transport protein Sec16A is a ~251 kDa protein. Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. Mediates the recruitment of MIA3/TANGO to ERES. Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane.

1. Watson, P. et al. (2006) Traffic (Copenhagen, Denmark) 7, 1678-87. PMID: 17005010
2. Bhattacharyya, D. et al. (2007) Molecular biology of the cell 18, 839-49. PMID: 17192411
3. Iinuma, T. et al. (2007) The Journal of biological chemistry 282, 17632-9. PMID: 17428803
4. Yonekawa, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12746-51. PMID: 21768384
5. Budnik, A. et al. (2011) Scientific reports 1, 77. PMID: 22355596
6. Maeda, M. et al. (2017) The Journal of cell biology 216, 1731-1743. PMID: 28442536
7. Piao, H. et al. (2017) Scientific reports 7, 39887. PMID: 28067262
8. Wu, Y. et al. (2018) PloS one 13, e0190407. PMID: 29300766
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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