Anti-USP12 Polyclonal Antibody (HB585014)

Name: Anti-USP12 Polyclonal Antibody
Brand: abinScience
SKU: HB585014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB585014

Description

Anti-USP12 Polyclonal Antibody (HB585014) is a rabbit polyclonal antibody detecting Ubiquitin carboxyl-terminal hydrolase 12; 3.4.19.12; Deubiquitinating enzyme 12; Ubiquitin thioesterase 12; Ubiquitin-hydrolyzing enzyme 1; Ubiquitin-specific-processing protease 12; USP12; UBH1; USP12L1 in ELISA, IHC, WB. Suitable for Human, Frog, Bovine, Mouse, and Zebrafish.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human USP12 (Ala20-Asp370).

Target

Ubiquitin carboxyl-terminal hydrolase 12; 3.4.19.12; Deubiquitinating enzyme 12; Ubiquitin thioesterase 12; Ubiquitin-hydrolyzing enzyme 1; Ubiquitin-specific-processing protease 12; USP12; UBH1; USP12L1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O75317, Q52KZ6, Q5M981, A5D9H7, Q9D9M2, A4FUN7, F1M625, P62068, A5WWB0, P62069

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 12 (USP12) is a ~42 kDa protein. Deubiquitinating enzyme that plays various roles in the regulation of the immune response and inflammation. During TCR engagement and activation, translocates into the cytoplasm and deubiquitinates its substrates LAT and TRAT1 and prevents their lysosome-dependent degradation to stabilize the TCR signaling complex at the plasma membrane. Plays an essential role in the selective LPS-induced macrophage response through the activation of NF-kappa-B pathway. In addition, promotes that antiviral immune response through targeting DNA sensor IFI16 to inhibit its proteasome-dependent degradation. Participates in the interferon signaling pathway and antiviral response independently of its deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels via inhibition of its CREBBP-mediated acetylation and subsequent dephosphorylation.

1. Cohn, MA. et al. (2009) The Journal of biological chemistry 284, 5343-51. PMID: 19075014

2. Li, H. et al. (2016) Molecular cell 63, 249-260. PMID: 27373336

3. Jahan, AS. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E705-14. PMID: 26811477

4. Nayak, TKS. et al. (2017) Biochemical and biophysical research communications 483, 69-74. PMID: 28063927

5. Fu, Y. et al. (2023) PLoS pathogens 19, e1011480. PMID: 37410794

6. Liu, J. et al. (2020) PLoS pathogens 16, e1008215. PMID: 31899788

7. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894

8. Moretti, J. et al. (2012) The Journal of biological chemistry 287, 29429-41. PMID: 22778262

9. Aron, R. et al. (2018) Nature communications 9, 3191. PMID: 30266909

10. Gangula, NR. et al. (2013) The Journal of biological chemistry 288, 34545-54. PMID: 24145035

Note

For research use only

Images

References

Datasheet

Anti-USP12 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-USP12 Polyclonal Antibody (HB585014)

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Datasheet

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