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Anti-RPS6 Polyclonal Antibody (HC002014)

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Overview
Catalog No.HC002014
Description
Anti-RPS6 Polyclonal Antibody (HC002014) is a rabbit polyclonal antibody detecting 40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein eS6; RPS6; OK/SW-cl.2 in ELISA, IHC, WB. Suitable for Bovine, Mouse, Human, Rat, and Rabbit.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityBovine, Mouse, Human, Rat, Rabbit, Chicken, Frog
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human RPS6 (Met1-Lys116).
Target 40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein eS6; RPS6; OK/SW-cl.2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5E995, P62754, P62753, P62755, G1TM55, P47838, P39017
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Small ribosomal subunit protein eS6 (RPS6) is a ~28 kDa protein. Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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