Anti-HTR2A Polyclonal Antibody (MB201014)

Name: Anti-HTR2A Polyclonal Antibody
Brand: abinScience
SKU: MB201014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

MB201014

Description

Anti-HTR2A Polyclonal Antibody (MB201014) is a rabbit polyclonal antibody detecting 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2A; Htr2a; Htr2 in ELISA, IHC, WB. Suitable for Mouse and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant mouse HTR2A (Glu2-Asn75&Lys385-Val471).

Target

5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2A; Htr2a; Htr2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P35363, P14842

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

5-hydroxytryptamine receptor 2A (HTR2A) is a ~52 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways.

1. Fiorica-Howells, E. et al. (2002) American journal of physiology. Gastrointestinal and liver physiology 282, G877-93. PMID: 11960784

2. Weisstaub, NV. et al. (2006) Science (New York, N.Y.) 313, 536-40. PMID: 16873667

3. González-Maeso, J. et al. (2007) Neuron 53, 439-52. PMID: 17270739

4. González-Maeso, J. et al. (2008) Nature 452, 93-7. PMID: 18297054

5. Albizu, L. et al. (2011) Neuropharmacology 61, 770-7. PMID: 21645528

6. Moreno, JL. et al. (2012) The Journal of biological chemistry 287, 44301-19. PMID: 23129762

7. Nelson, PM. et al. (2012) Experimental diabetes research 2012, 398406. PMID: 23346101

Note

For research use only

Images

References

Datasheet

Anti-HTR2A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HTR2A Polyclonal Antibody (MB201014)

Overview

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References

Datasheet

SDS

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