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Anti-FDX1 Polyclonal Antibody (MY268014)

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Overview
Catalog No.MY268014
Description
Anti-FDX1 Polyclonal Antibody (MY268014) is a rabbit polyclonal antibody detecting mitochondrial; Adrenal ferredoxin; Ferredoxin-1; Fdx1 in ELISA, IHC, WB. Suitable for Rat, Human, Mouse, Bovine, and Chicken.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mouse FDX1 (Glu68-Ser188).
Target Adrenodoxin, mitochondrial; Adrenal ferredoxin; Ferredoxin-1; Fdx1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P00258, P46656, P10109, P00257, P29330, P24483, P13216
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adrenodoxin, mitochondrial (FDX1) is a ~19 kDa protein. Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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