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Anti-Isocitrate lyase Polyclonal Antibody (JN930014)

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Overview
Catalog No.JN930014
Description
Anti-Isocitrate lyase Polyclonal Antibody (JN930014) is a rabbit polyclonal antibody detecting Isocitrate lyase; ICL; 4.1.3.1; Isocitrase; Isocitratase; icl; Rv0467; MTV038.11 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMycobacterium tuberculosis
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Isocitrate lyase (Met1-His428).
Target Isocitrate lyase; ICL; 4.1.3.1; Isocitrase; Isocitratase; icl; Rv0467; MTV038.11
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P9WKK6, P9WKK7, H8EVV4, P0A5H4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Isocitrate lyase 1 is a ~47 kDa protein. Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It also catalyzes the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Isocitrate lyase Polyclonal Antibody

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