Anti-MERS-CoV nsp5 Polyclonal Antibody (VK072054)

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Overview
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Datasheet

Overview

Catalog No.

VK072054

Description

Anti-MERS-CoV nsp5 Polyclonal Antibody (VK072054) is a rabbit polyclonal antibody detecting nsp5 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant MERS-CoV Replicase polyprotein 1a (Ser3248-Gln3553).

Target

Replicase polyprotein 1a, pp1a, ORF1a polyprotein, Host translation inhibitor nsp1, nsp1, Leader protein, Non-structural protein 2, nsp2, p65 homolog, Papain-like protease nsp3, PL-PRO, EC:3.4.19.12, EC:3.4.22.-, Non-structural protein 3, nsp3, PL2-PRO, Non-structural protein 4, nsp4, 3C-like proteinase nsp5, 3CL-PRO, 3CLp, EC:3.4.22.69, nsp5, Non-structural protein 6, nsp6, Non-structural protein 7, nsp7, Non-structural protein 8, nsp8, RNA-capping enzyme subunit nsp9, Non-structural protein 9, nsp9, EC:2.7.7.50, Non-structural protein 10, nsp10, Growth factor-like peptide, GFL, Non-structural protein 11, nsp11

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

K9N638

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Replicase polyprotein 1a (nsp5) is a ~486 kDa protein. The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

Note

For research use only

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References

Datasheet