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Anti-MRV-1 SigmaNS/S3 Polyclonal Antibody (VK093014)

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Overview
Catalog No.VK093014
Description
Anti-MRV-1 SigmaNS/S3 Polyclonal Antibody (VK093014) is a rabbit polyclonal antibody detecting MRV-1 SigmaNS/S3 in ELISA, IHC, WB. Suitable for Reovirus type 1, Reovirus type 2, and Reovirus type 3.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMammalian orthoreovirus 1 (MRV1)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant MRV-1 SigmaNS/S3 (Met1-Val366).
Target sigma-NS protein, S3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P07940, P12002, P03526
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein sigma-NS (SigmaNS/S3) is a ~41 kDa protein. Protein that binds to ssRNA and participates with protein mu-NS in forming the matrix of viral factories, which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Plays a role in the inhibition of the integrated stress response (ISR) to escape from host cell translational shutoff. Participates in the disruption of stress granules (SG) through its association with host G3BP1 and mu-NS.

1. Gillian, AL. et al. (1998) Virology 240, 1-11. PMID: 9448684
2. Choudhury, P. et al. (2017) Journal of virology 91. PMID: 28794026
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-MRV-1 SigmaNS/S3 Polyclonal Antibody

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