Anti-ZEBOV VP35 Polyclonal Antibody (VK661014)

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Datasheet

Overview

Catalog No.

VK661014

Description

Anti-ZEBOV VP35 Polyclonal Antibody (VK661014) is a rabbit polyclonal antibody detecting VP35 in ELISA, IHC, WB. Suitable for Reston ebolavirus, Zaire ebolavirus, and Sudan ebolavirus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Reston ebolavirus, Zaire ebolavirus, Sudan ebolavirus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ZEBOV VP35 (Gly215-Ile340).

Target

Polymerase cofactor VP35, Ebola VP35, eVP35, VP35

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q8JPY0, Q5XX07, Q05127, Q6V1Q9, Q91DE0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Polymerase cofactor VP35 is a ~36 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1.

1. Kimberlin, CR. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 314-9. PMID: 20018665

Note

For research use only

Images

References

Datasheet

Anti-ZEBOV VP35 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ZEBOV VP35 Polyclonal Antibody (VK661014)

Overview

Images

References

Datasheet

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