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Anti-NS3 Polyclonal Antibody (VK089024)

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Overview
Catalog No.VK089024
Description
Anti-NS3 Polyclonal Antibody (VK089024) is a rabbit polyclonal antibody detecting Serine protease NS3 in ELISA, IHC, WB. Suitable for Louping ill virus and Langat virus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityLouping ill virus (LIV), Tick-borne encephalitis virus (TBEV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant LIV NS3 (Ala1671-Gly1967).
Target Genome polyprotein, Capsid protein C, Core protein, Protein prM, Peptide pr, Small envelope protein M, Matrix protein, Envelope protein E, Non-structural protein 1, NS1, Non-structural protein 2A, NS2A, Serine protease subunit NS2B, Flavivirin protease NS2B regulatory subunit, Non-structural protein 2B, Serine protease NS3, 3.4.21.91, 3.6.1.15, 3.6.4.13, Flavivirin protease NS3 catalytic subunit, Non-structural protein 3, Non-structural protein 4A, NS4A, Peptide 2k, Non-structural protein 4B, NS4B, RNA-directed RNA polymerase NS5, 2.1.1.56, 2.1.1.57, 2.7.7.48, Non-structural protein 5
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P22338, Q01299, D7RF80, P29837, Q91B85, Q04538, Q7T6D2, P14336, P07720
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Genome polyprotein (NS3) is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the host endoplasmic reticulum (ER) membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. The capsid protein initially binds electrostatically to lipids with negatively-charged head groups. Once membrane-bound, the capsid protein is able to recruit the viral genomic RNA onto the ER membrane.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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