Anti-Human VMAT2 Antibody (JP1212) (HC560063)

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Overview

Catalog No.	HC560063
Description	Anti-Human VMAT2 Antibody (JP1212) [JP1212] (HC560063) is a human monoclonal antibody detecting VMAT2 in FCM. Suitable for Human, Mouse, Pig, and Rat. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Multi-Species — Cross-reactive for translational research.
Species reactivity	Human, Mouse, Pig, Rat
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	JP1212
Clonality	Monoclonal
Target	Synaptic vesicular amine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, VAT2, Vesicular monoamine transporter 2, SLC18A2, SVMT, VMAT2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q05940, Q8BRU6, Q01827, Q01827, P54219, Q8R090, Q01818
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Synaptic vesicular amine transporter (VMAT2) is a ~55 kDa protein. Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin. Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals. 1. Rilstone, JJ. et al. (2013) The New England journal of medicine 368, 543-50. PMID: 23363473 2. Pidathala, S. et al. (2023) Nature 623, 1086-1092. PMID: 37914936 3. Wu, D. et al. (2024) Nature 626, 427-434. PMID: 38081299 4. Dalton, MP. et al. (2024) eLife 12. PMID: 38517752 5. Erickson, JD. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 5166-71. PMID: 8643547 6. Erickson, JD. et al. (1995) Journal of molecular neuroscience : MN 6, 277-87. PMID: 8860238
Note	For research use only

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Flow cytometry

HEK293T cells transiently transfected with human, mouse, rat, or pig VMAT1 or VMAT2 were stained with clone JP1212 at the indicated concentrations (0.01, 0.04, 0.16, 0.62, 2.5, and 10 µg/mL). The antibody recognized both VMAT1 and VMAT2 across all four species tested.
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Binding affinity

References

Datasheet

Anti-Human VMAT2 Antibody (JP1212).pdf

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Anti-Human VMAT2 Antibody (JP1212) (HC560063)

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