Anti-HRV-14 VP1 Polyclonal Antibody (VK102044)

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Datasheet

Overview

Catalog No.

VK102044

Description

Anti-HRV-14 VP1 Polyclonal Antibody (VK102044) is a rabbit polyclonal antibody detecting 1D (VP1) in ELISA, IHC, WB. Suitable for Human rhinovirus 14.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human rhinovirus 14

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HRV-14 VP1 (Gly1-Tyr289).

Target

1D (VP1), 1D (VP1)

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P03303

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (VP1) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host ICAM1 to provide virion attachment to target host cells. This attachment induces virion internalization.

1. Kolatkar, PR. et al. (1999) The EMBO journal 18, 6249-59. PMID: 10562537

2. Dong, Y. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 8017-8022. PMID: 28696310

Note

For research use only

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References

Datasheet