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Anti-MHV-4 Nucleocapsid Polyclonal Antibody (VK107034)

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Overview
Catalog No.VK107034
Description
Anti-MHV-4 Nucleocapsid Polyclonal Antibody (VK107034) is a rabbit polyclonal antibody detecting nucleocapsid protein in ELISA, IHC, WB. Suitable for Rat coronavirus and Murine coronavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMurine coronavirus (MHV-4) (Murine hepatitis virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant MHV-4 Nucleocapsid (Met1-Val455).
Target nucleocapsid protein, nucleocapsid protein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q83360, P59713, P18447, P03417, Q9PY96, Q02915, P18448, P03416, P18446, Q9WCD0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein (Nucleocapsid) is a ~49 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-MHV-4 Nucleocapsid Polyclonal Antibody

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