Anti-Human NMI Polyclonal Antibody (HD442014)

Name: Anti-Human NMI Polyclonal Antibody
Brand: abinScience
SKU: HD442014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HD442014

Description

Anti-Human NMI Polyclonal Antibody (HD442014) is a rabbit polyclonal antibody detecting NMI in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NMI (Phe58-Glu307).

Target

N-myc and STAT interactor, N-myc-interactor, NMI, Nmi

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q13287

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Q13287

Background

N-myc-interactor (NMI) is a ~35 kDa protein. Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21.

1. Das, A. et al. (2015) Virology 485, 383-92. PMID: 26342464

2. Xiahou, Z. et al. (2017) Nature communications 8, 950. PMID: 29038465

3. Jian, D. et al. (2018) Acta physiologica (Oxford, England) 223, e13037. PMID: 29350881

4. Zhu, M. et al. (1999) Cell 96, 121-30. PMID: 9989503

5. Feng, L. et al. (2018) PLoS pathogens 14, e1006867. PMID: 29377960

6. Zhou, X. et al. (2000) The Journal of biological chemistry 275, 21364-71. PMID: 10779520

7. Chen, J. et al. (2000) The Journal of biological chemistry 275, 36278-84. PMID: 10950963

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NMI polyclonal antibody at 1ug/mL on various samples.
Lane 1: PC-3 cell lysate

References

Datasheet

Anti-Human NMI Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NMI Polyclonal Antibody (HD442014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us