Anti-Human HTATSF1 Polyclonal Antibody (HD661014)

Name: Anti-Human HTATSF1 Polyclonal Antibody
Brand: abinScience
SKU: HD661014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HD661014

Description

Anti-Human HTATSF1 Polyclonal Antibody (HD661014) is a rabbit polyclonal antibody detecting HTATSF1. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HTATSF1 (Phe125-Ser389).

Target

17S U2 SnRNP complex component HTATSF1, HIV Tat-specific factor 1, HTATSF1, Tat-SF1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O43719

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

17S U2 SnRNP complex component HTATSF1 is a ~85 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint-interacting stem loop. HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences. Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation.

1. Loerch, S. et al. (2019) The Journal of biological chemistry 294, 2892-2902. PMID: 30567737

2. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

3. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

4. Kim, JB. et al. (1999) Molecular and cellular biology 19, 5960-8. PMID: 10454543

Note

For research use only

Images

References

Datasheet

Anti-Human HTATSF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human HTATSF1 Polyclonal Antibody (HD661014)

Overview

Images

References

Datasheet

SDS

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