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Anti-Human DNAJC2 Polyclonal Antibody (HV184014)

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Anti-Human DNAJC2 Polyclonal Antibody
Anti-Human DNAJC2 Polyclonal Antibody
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Overview
Catalog No.HV184014
Description
Anti-Human DNAJC2 Polyclonal Antibody (HV184014) is a rabbit polyclonal antibody detecting DNAJC2. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DNAJC2 (Lys442-Lys621).
Target DNAJC2, DnaJ homolog subfamily C member 2, DnaJ homolog subfamily C member 2, N-terminally processed, M-phase phosphoprotein 11, MPHOSPH11, MPP11, ZRF1, Zuotin-related factor 1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q99543
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

DnaJ homolog subfamily C member 2 (DNAJC2) is a ~71 kDa protein. Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation.

Note For research use only
Images
  • Anti-Human DNAJC2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-DNAJC2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Caco2 cell lysate
    Lane 2: HT-29 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: K562 cell lysate
    Lane 6: PC-3 cell lysate
    Lane 7: NIH3T3 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DNAJC2 Polyclonal Antibody

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