Anti-Human CLNS1A Polyclonal Antibody (HD609014)

Name: Anti-Human CLNS1A Polyclonal Antibody
Brand: abinScience
SKU: HD609014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HD609014

Description

Anti-Human CLNS1A Polyclonal Antibody (HD609014) is a rabbit polyclonal antibody detecting CLNS1A. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CLNS1A (Met1-Leu135).

Target

CLCI, CLNS1A, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, Chloride ion current inducer protein, ClCI, I(Cln), ICLN, Methylosome subunit pICln, Reticulocyte pICln

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P54105

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Methylosome subunit pICln (CLNS1A) is a ~26 kDa protein. Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.

1. Pu, WT. et al. (1999) Molecular and cellular biology 19, 4113-20. PMID: 10330151

2. Friesen, WJ. et al. (2001) Molecular and cellular biology 21, 8289-300. PMID: 11713266

3. Chari, A. et al. (2008) Cell 135, 497-509. PMID: 18984161

4. Guderian, G. et al. (2011) The Journal of biological chemistry 286, 1976-86. PMID: 21081503

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CLNS1A polyclonal antibody at 1ug/mL on various samples.
Lane 1: HT-29 cell lysate
Lane 2: 293T cell lysate
Lane 3: Hela cell lysate

References

Datasheet

Anti-Human CLNS1A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human CLNS1A Polyclonal Antibody (HD609014)

Overview

Images

References

Datasheet

SDS

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