Anti-Human SMN1 Polyclonal Antibody (HS868014)

Name: Anti-Human SMN1 Polyclonal Antibody
Brand: abinScience
SKU: HS868014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HS868014

Description

Anti-Human SMN1 Polyclonal Antibody (HS868014) is a rabbit polyclonal antibody detecting SMN1. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SMN1 (Met1-Asn294).

Target

Component of gems 1, Gemin-1, SMN, SMN1, SMNT, Survival motor neuron protein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q16637

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Survival motor neuron protein (SMN1) is a ~31 kDa protein. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits.

1. Chari, A. et al. (2008) Cell 135, 497-509. PMID: 18984161

2. Pellizzoni, L. et al. (1998) Cell 95, 615-24. PMID: 9845364

3. Otter, S. et al. (2007) The Journal of biological chemistry 282, 5825-33. PMID: 17178713

4. Zhang, R. et al. (2011) Cell 146, 384-95. PMID: 21816274

5. Tripsianes, K. et al. (2011) Nature structural & molecular biology 18, 1414-20. PMID: 22101937

6. Yi, H. et al. (2020) Nucleic acids research 48, 895-911. PMID: 31799625

7. Lotti, F. et al. (2012) Cell 151, 440-54. PMID: 23063131

8. Zhao, DY. et al. (2016) Nature 529, 48-53. PMID: 26700805

Note

For research use only

Images

References

Datasheet

Anti-Human SMN1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human SMN1 Polyclonal Antibody (HS868014)

Overview

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References

Datasheet

SDS

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