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Anti-Magnaporthe oryzae ATG8 Polyclonal Antibody (JN177014)

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Overview
Catalog No.JN177014
Description
Anti-Magnaporthe oryzae ATG8 Polyclonal Antibody (JN177014) is a rabbit polyclonal antibody detecting ATG8. Suitable for Phaeosphaeria nodorum, Sclerotinia sclerotiorum, Aspergillus oryzae, Coccidioides immitis, Glomerella lagenarium, Aspergillus fumigatus, Pyricularia oryzae, Colletotrichum lindemuthianum, Neurospora crassa, Aspergillus terreus, Gibberella zeae, Penicillium rubens, Botryotinia fuckeliana, Beauveria bassiana, Neosartorya fischeri, Aspergillus clavatus, Podospora anserina, Emericella nidulans, Cochliobolus heterostrophus, Chaetomium globosum, and Aspergillus niger.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityPhaeosphaeria nodorum, Sclerotinia sclerotiorum, Aspergillus oryzae, Aspergillus niger
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Magnaporthe oryzae ATG8 (Met1-Phe115).
Target ATG8, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q51MW4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Autophagy-related protein 8 (ATG8) is a ~14 kDa protein. Ubiquitin-like modifier involved in autophagosome formation. With ATG4, mediates the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress.

Note For research use only
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Formula
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Formula
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Magnaporthe oryzae ATG8 Polyclonal Antibody

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