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Anti-Mouse AGRN Polyclonal Antibody (MT005014)

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Overview
Catalog No.MT005014
Description
Anti-Mouse AGRN Polyclonal Antibody (MT005014) is a rabbit polyclonal antibody detecting Agrn. Suitable for Rat and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse AGRN (Val1256-Gly1426).
Target Agrin, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 90 kDa fragment, Agrin N-terminal 110 kDa subunit, Agrn, C22, C90
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession A2ASQ1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Agrin (AGRN) is a ~207 kDa protein. Depending on alternative splicing and post-translational modifications, it has a role in different processes, including neuromuscular junction formation and maintenance, and regulation of neurite outgrowth. Involved in the positive regulation of cartilage formation through alpha-dystroglycan binding and up-regulation of SOX9.

1. Burgess, RW. et al. (1999) Neuron 23, 33-44. PMID: 10402191
2. Burgess, RW. et al. (2000) The Journal of cell biology 151, 41-52. PMID: 11018052
3. Hoover, CL. et al. (2003) The Journal of cell biology 161, 923-32. PMID: 12796478
4. Ksiazek, I. et al. (2007) The Journal of neuroscience : the official journal of the Society for Neuroscience 27, 7183-95. PMID: 17611272
5. Matsumoto-Miyai, K. et al. (2009) Cell 136, 1161-71. PMID: 19303856
6. Bütikofer, L. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 4378-93. PMID: 21885656
7. Glass, DJ. et al. (1996) Cell 85, 513-23. PMID: 8653787
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse AGRN Polyclonal Antibody

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