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Anti-Mouse IL3 Polyclonal Antibody (MY262014)

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Overview
Catalog No.MY262014
Description
Anti-Mouse IL3 Polyclonal Antibody (MY262014) is a rabbit polyclonal antibody detecting IL3. Suitable for Mouse.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse IL3 (Asp33-Cys166).
Target Hematopoietic growth factor, IL-3, IL3, Interleukin-3, MCGF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P01586
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Interleukin-3 (IL3) is a ~18 kDa protein. Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB.

1. Jaster, R. et al. (1999) Cellular signalling 11, 331-5. PMID: 10376805
2. Tong, Y. et al. (2020) JCI insight 5. PMID: 31990690
3. Silvennoinen, O. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 8429-33. PMID: 8378315
Note For research use only
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Formula
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Anti-Mouse IL3 Polyclonal Antibody

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