Anti-Human TRMT5 Polyclonal Antibody (HD270014)

Name: Anti-Human TRMT5 Polyclonal Antibody
Brand: abinScience
SKU: HD270014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD270014

Description

Anti-Human TRMT5 Polyclonal Antibody (HD270014) is a rabbit polyclonal antibody detecting TRMT5. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRMT5 (Thr65-Lys475).

Target

EC:2.1.1.228, KIAA1393, M1G-methyltransferase, TRM5, TRMT5, tRNA (guanine(37)-N(1))-methyltransferase, tRNA [GM37] methyltransferase, tRNA methyltransferase 5 homolog

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q32P41

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

tRNA (guanine(37)-N(1))-methyltransferase (TRMT5) is a ~58 kDa protein. Involved in mitochondrial tRNA methylation. Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

1. Powell, CA. et al. (2015) American journal of human genetics 97, 319-28. PMID: 26189817

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-TRMT5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-Human TRMT5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TRMT5 Polyclonal Antibody (HD270014)

Overview

Images

References

Datasheet

SDS

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